This study was carried out to investigate the effects of different sources and levels of dietary lipid on lecithin : cholesterol acyltrasferase activity and cholesterol metabolism in male rats of Sprague-Dawley strain. The effects of different
lipid
sources was compared with sardine oil(φ3 EPA and DHA), beef tallow(SFA). Perilla oil(φ3 linolenic acid) and corn oil(φ6 linoleic acid). Diets were formaulted in such a way that 10%, 20% and 40% dietary energy were supplied with each of four
experimental lipid supplied with each of four experimental lipid sources. Control diet contained only non-lipid energy. A total number of 78 rats, equally divided into 13 groups, were fed the experimental diets for a period of 6 weeks. In vitro
cultures
were. In vitro cultures were also carried out to study the cholesterol synthetic activity in the ,liver prepared from rats used in feeding trials.
The concentration of plasma total cholesterol. HDL-cholesterol, LDL-cholesterol and HDL-C/T-C(total cholesterol) ratio were significantly(p<0.001) influenced by dietary lipid sources. Higher HDL-cholesterol and lower LDL-cholesterol concentration
in
plasma were obtained in rats fed φ3 fatty acid supplemented diets(sardine oil and perilla oil group)compared to diets containing φ6 and saturated fatty acid(corn oil and beef tallow group). In total cholesterol concentration of plasma, beef
tallow
group was significantly(p<0.001) higher than other lipid groups, and non-lipid group was significantly(p<0.05) higher than the lipid supplemented groups.
The activity of lecithin : cholesterol acyltransferase(LCAT) in plasma was greatly(p<0.001) affected by dietary lipid sources and levels. In LCAT activity of plasma, lipid supplemented groups were signiicanlty(p<0.05) higher than non-lipid group,
vegetable oil groups were significantly(p<0.001) higher than beef tallow group. Also perilla oil groups was significantly(p<0.05) higher than beef tallow group. also perilla oil groups was significantly(p<0.05) higher than perilla oil group. Low
lipid
group, compared with medium or high lipid group, showed higher activity of LCAT in plasma.
In cholesterol synthetic activity of liver tissues culture, sardine oil group(φ3 EPA and DHA) was significantly(p<0.0001) higher than other lipid groups, non-lipid was significantly(p<0.001) higher than the lipid supplemented groups, and amimal
fat
group were significantly(p<0.001) higher than vegetable oil groups, but the synthetic activity was not affected by dietary lipid levels.
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